International Journal of Innovative Horticulture
Year : 2012, Volume : 1, Issue : 1
First page : ( 63) Last page : ( 67)
Print ISSN : 2320-0286. Online ISSN : 2582-2527.

Inter Simple Sequence Repeat (ISSR) markers for identification of germplasm and diversity analysis in coconut (Cocos nucifera L.)

Manimekalai R.*

Central Plantation Crops Research Institute, Kasaragod – 671124

*Corresponding author: rmanimekalaiicar@gmail.com

Online published on 4 December, 2019.

Abstract

Inter Simple Sequence Repeat (ISSR) is a powerful molecular marker technique used for variety of purposes like genetic diversity analysis, phylogeny information, germplasm identification, gene tagging etc. In the present paper, 33 coconut (Cocos nucifera L.) germplasm representing different geographical regions have been fingerprinted with highly polymorphic ISSR markers. Ten polymorphic primers targeting the microsatellite regions were used to amplify the coconut germplasms. Ten primer produced 104 polymorphic markers with 87 percent polymorphism. Cluster analysis was carried out among 33 accessions by UPGMA using the software POPGENE ver.1.32. The dendrogram revealed five clusters. Based on the fingerprint data germplasm specific markers were identified using the primers UBC 810, UBC 915, UBC 823, UBC 824 and UBC 855. The marker UBC 815₁₆₇₃ was specific to Panama Tall (PNT) and Saint Vincent Tall (STVT). The marker UBC 854₅₇₃ was specific to Nuwehnug Tall (NWHT). ISSR markers were proved to be useful for generating fingerprints and for deriving genetic relationships in coconut.

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Keywords

Coconut, ISSR markers, germplasm, specific markers.

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