Mutation studies on Streptomyces for amphotericin b yield improvement Upadhyaya Anitha1,*, Nagasatya A2, Thajuddin N3, Paneerselvam A4, Giridhara Upadhyaya5 1Department of Microbiology, J J College of Arts and Science, Pudukkottai, TN, India 2Department of Zoology, Govt Arts College for women, Pudukkottai, Tamilnadu, India 3Department of Microbiology, Bharathidasan University, Tiruchirapalli-24, Tamilnadu 4P.G. and Research Department of Botany, Microbiology, A.V.V.M. Sri Pushpam College (Automomous), Poondi, Tamilnadu, India 5Kempegowda Institute of Medical sciences, BSK 2nd stage, Bangalore, India *Corresponding Author: E-mail: ani_giru@yahoo.com
Online published on 21 March, 2015. Abstract Four Streptomyces strains isolated from the soil of Western Ghats of India producing Amphotericin B were subjected to random mutagenesis by UV irradiations for yield improvement. The mutants were then screened for their Amphotericin B concentrations and their antifungal activity by agar well diffusion method. Two mutants demonstrated 13% and 16% higher concentration than the isolated strains respectively. Thus mutation can be a very useful tool to minimize production cost with the same benefits. Top Keywords Amphotericin B, UV mutation, Streptomyces, strain improvement. Top |