Indian Journal of Nematology
Year : 1981, Volume : 11, Issue : 2
First page : ( 180) Last page : ( 188)
Print ISSN : 0303-6960.

Protein patterns in resistant and susceptible tomato varieties inoculated with the root-knot nematode Meloidogyne incognita

Ganguly Sudershan, Dasgupta D. R.

Divisian of Nematalagy, Indian Agricultural Research Institute, New Delhi-110012.

Received:  January,  1981.

Abstract

Proteins and peroxidase activity were determined in healthy and Meloidogyne incognita—inoculated tomato varieties Pusa Ruby, SL-120 and Nematex. Vars. Nematex and SL-120 showed resistant reactions, and var. Pusa Ruby was susceptible to the nematode. Increased protein levels were observed in the diseased tissues of all the three vars., 28 days after inoculation. Quantitative changes in protein concentrations were accompanied with qualitative alterations in protein patterns. The increases in the peroxidase activity in the inoculated plants of all the three vars. were sharp, and more conspicuous in the resistant-reacting vars. The qualitative changes fn peroxidase activity were characterized by (i) formation of new components only after nematode infestation of host tissues (ii) increased or decreased intensity of newly formed isozymes (iii) changed intensity of pre-existing components and (iv) disappearance of some components after the parasitic invasion of host tissues. Contrary to the resistant var., the inoculated susceptible var. failed to restore the shift in enzymic balance, 28 days after inoculation.

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