Study of MRSA and ESBL Organisms Isolated from Infected Wounds Suresh P.1,*, Reddy V. Sreenivasulu2, Kumar V. Praveen1, Krishna P. Vamsimuni1 1PH.D Scholar, Bharath University, Thambram, Chennai 2Professor, Department of Microbiology, Srilaxmi Narayana Institute of Medical Sciences (SRLIMS) Osudu, Puducherry, India *Corresponding Author: Suresh P PH.D Scholar Bharath University Thambram, Chennai Email: sureshraja25@gmail.com
Online published on 21 February, 2019. Abstract Background Recent trends in Extended spectrum β-lactamase(ESBL)&MR Producing bacterial pathogen causes various life threatening infections lead to sepsis related Mortality. The ESBLs can makes the infection caused by the bacteria much harder to treat. Method pus Aspiration taken from various anatomical sites of wound area was collected using sterile swab under aseptic Conditions and further processed by doing Grams stain and culture. Culture was done on Blood agar, Brain Heart Infusion agar, Mac Conkeys agar, incubated at 370c for 24hrs. Isolates confirmed by biochemical tests, antibiotic susceptibility testing was done using muller Hinton agar by Kirby Bauer's method as per standard CLSI guidelines. Results out of 396 pus samples, 236 were culture positive. Most common organisms isolated was MRSA 81(34.3%) followed by MRCons & Other isolates included were , ESBL E.coli, ESBL Klebsiella. and Pseudomonas. Conclusion Present study observed bacteria causing wound infection. High Percentage of bacterial growth were seen in samples drawn from surgery wards and dialasis ward, labour ward followed by orthopedics. S.aureus (34.3%)was the predominant isolated followed by MRCoNS (15.8%), E.coli(10.5%). MRSA incidence in our study was 10% and ESBL's detected in 20% of E.coli strains and 6.6% Klebsiella strains. Top Keywords Wound infection, Pus, MRSA, ESBL E.coli & Klebsiella. Top |