Failure of Speed oligo Mycobacteria to diagnose Mycobacterium tuberculosis Complex Directly from Sputum Samples Alnour Tarig MS1,7,*, Abuduhier Faisel2, Abakur Eltayib H Ahmed3,8, Albalawi Fahad MA4, Alfifi Khalid AS5, Silvala Bernard C.6 1Assistant Professor, Faculty of Applied Medical Sciences, Department of Medical Laboratory Technology, University of Tabuk 2Associate professor Department of Medical Laboratory Technology, Dean of Academic affairs-University of Tabuk 3Associate professor, Faculty of Applied Medical Sciences, Department of Medical Laboratory Technology 4Medical Laboratory Specialist, Laboratory Department-King Fahad Specialist Hospital 5Medical Laboratory Specialist, Laboratory Department-King Fahad Specialist Hospital 6Assistant professor-Faculty of Applied Medical Sciences, Department of Medical Laboratory Technology, University of Tabuk 7Assistant professor-Faculty of Medical Laboratory Sciences-Department of Microbiology, Alzaiem AlAzhari University Khartoum North-Sudan 8Associate professor Faculty of Medical Laboratory Sciences, Department of Microbiology, Alzaiem AlAzhari University *Corresponding Author: Tarig MS Alnour Assistant Professor, Faculty of Applied Medical Sciences, University of Tabuk P.O Box: 741 Tabuk: 71491 Faculty of Medical Laboratory Sciences, Department of Microbiology and Immunology AlZaiem AlAzhari University, Khartoum North-Sudan Phone: +966535782141 Email: telnour@ut.edu.sa
Online published on 21 February, 2019. Abstract Introduction Rapid and accurate diagnosis of tuberculosis facilitate treatment outcome and reduce the frequency of drug resistance. This study aimed to determine the role of speed-Oligo Mycobacteria in the rapid diagnosis and characterization of Mycobacteria to the species level. Method A total number of 46 confirmed positive tuberculosis sputum samples by smear microscopy, conventional PCR with IS6110 and Genotype mycobacterium CM were tested by Speed-Oligo Mycobacteria. Results All tested samples showed No results with speed-Oligo Mycobacteria, while a positive with Genotype Mycobacterium CM and conventional PCR. Conclusion Our finding oppose the use of speed-oligo mycobacteria for direct diagnosis of TB from clinical samples, recommending the use of conventional PCR method, Genotype, GeneXpert or any other molecular techniques used for direct diagnosis of TB from clinical specimens. Top Keywords Speed oligo Mycobacteria, Mycobacterium tuberculosis, Tuberculosis, Sputum sample, Genotype Mycobacterium CM. Top |