Estimation of HSV-1 and HSV-2 in Chronic Periodontitis in Babylon Subjects Alkafaji Younis Abdulridha K.1,*, Kzar Mahdi Yakoob2, AL-deen Zahraa Dhafer Shams3 1Medical Microbiology Department, College of Dentistry, Babylon University, Hilla, Iraq 2Maxillofacial Surgery Department, College of Dentistry Babylon University, Hilla, Iraq 3College of Dentistry, Al-Kufa University, Al-Najaf, Iraq *Corresponding author: Younis Abdulridha K. Alkafaji. Medical Microbiology Department, College of Dentistry, Babylon University, Hilla, Iraq
Online published on 21 February, 2019. Abstract This study was carried to detect the prevalence of HSV-1 and HSV-2 in periodontal pocket of healthy and chronic periodontitis infected subjects of Babylon province/AL-Hilla city by using Real Time-Polymerase Chain Reaction technique with the amplification of Major capsid protein gene which is responsible for virulence of the these viruses as specified primer. Ethical approval and prior consent were taken. Sixty-eight subjects (21–79 years old) mean (43.95±11.79) years, included29 males and 39 females. Supragingival plaque samples were removed. Samples of gingival crevicular fluid were collected from all participants, 38 from chronically infected periodontal patients and 30 from healthy periodontal subjects. Seven (18.42%) out of 38 samples ofchronically infected subjectsappeared positive result of HSV-1. While10(26.32%) samples revealed positive result of HSV-2 in the same group. Whereas in healthy periodontal groupshownsix (20.00%) subjects gave positive result of HSV-1 and 5(16.67%) subjects exposed positive result of HSV-2. The coinfection of HSV-1 and HSV-2 was found in two (5.26%) of chronic periodontitis patients and in two (6.67%) of healthy subjects. Top Keywords HSV-1, HSV-2, chronic periodontitis. Top |