Molecular & Antibiotic Sensitivity Pattern Study of Acute Otitis Media, Tonsillitis & Periodontitis among Different Ages of Patients of Al-Hashmia General Hospital, Primary Care Centers & Private Clinic in Babylon Province Saad Ali Malik1,*, Aljarah Ali Kadhim2, Alkhafaji Nebras Mohammed Sahi1, Kadhim Wurood Alwan1 1Biology Department, College of Science for Women, Babylon University, Iraq 2Branch of Microbiology, College of Veterinary Medicine, University of Kerbala, Iraq *Corresponding author: Ali Malik Saad., Biology Department, College of Science for Women, Babylon University, Iraq
Online published on 21 February, 2019. Abstract A total of 89 specimens have included 34 swabs of periodontitis(13male, 21 female), 28 swabs of Otitis Media & 27 swabs of Tonsillitis. These samples were brought from patients consulted to Alhashmia Hospital, primary care centers & private clinic of Babylon province during the period from 5 October 2017 to 11December 2017. They were appeared (72.17)% positive cases for Streptococcus pyogenes which have gotten from patients within age ranged, which suffered from Ear infection (3 to 39 years), Tonsillitis(1 to 60 years) & periodontitis inflammation(from less than 3years to 60 years). Biochemical examinations have included oxidase, catalase & Antibiotics sensitivity test. Results of bacterial genetics detection of sic as responsible for virulence agents of isolates bacteria, which is responsible for damage host cell components. 50 patterns (55.3%) of S. pyogenes cases of otitis media, tonsillitis & gingivitis have positive exclude 39(44.7) isolates were negative on 3% agarose gel. The volume of sic gene was 150 bp of the DNA, ladder (100–1000)bp. This gene of S. pyogenes specimens has amplified by PCR utilizing specific forward & reverse primers have appeared that 50 Streptococcus isolates detection of Otitis Media., Tonsillitis & periodontitis infections have negative 27 (90%) except 2 (10%) isolates were positive. Top Keywords Sic gene, S. pyogenes, Acute Otitis Media, Tonsillitis & Periodontitis. Top |