Determination of Some Human Enzymes Activities in Presence of Titanium Oxide Nanoparticles Abdullah Amer Hasan1, Hammoodi Manal Sadiq2, Eltayef Emad Mahmoud3, Safaryan Abeer H.M.4,* 1Assist. Prof., Ph. D., Chemistry Department, Science Collage, Mustansiriyah University 2M. Sc., Chemistry Department, Science Collage, Mustansiriyah University 3M. Sc., Chemistry Department, Science Collage, Mustansiriyah University 4Assist. Prof., Ph. D., Chemistry Department, Science Collage, Mustansiriyah University *Corresponding Author: Dr. Abeer Hazim Mustafa Safaryan, Chemistry Department, Science Collage, Mustansiriyah University, Bagdad, Iraq, e-mail: ahs1022@uomustansiriyah.edu.iq
Online published on 23 December, 2019. Abstract Titanium oxide nanoparticles (TiO2NPs) are the most common particles that have industrial, commercial, medial and antibacterial applications. On the other hand, they have toxic properties within some concentrations that show the ability to inhibit some human enzymes. In this study a diluted solutions of TiO2NPs were prepared by weighting(0.08 gm)with deionized water as(10−1, 10−2, 10−3, 10−4, 10−5) with low conductivity to avoid interaction with other ions. Our study aimed to estimate the effect of different concentration of TiO2NPs on liver enzymesaspartate transaminase (GOT), alanine transaminase (GPT), alkaline phosphatase(ALP) and Amylase in human serum by using a healthy human serum with a mixture of enzyme solution in absence and presence of diluted solution of TiO2NPs. The results showed that different TiO2NPs concentrations used had inhibitory effect on all the enzymes. With TiO2NPs high concentration solutions the activity of enzyme decreased, inversely and the efficiency inhibition ratio increase. The kinetic properties of enzymes in presence of TiO2NPs solutions had been studied as well. Different concentrations of substrate used to study type of inhibition. The study illustrated that there is a competitive inhibition in GOT and ALP enzymes, while there was uncompetitive inhibition in case of GPT and Amylase enzymes. Transmission electron microscope (TEM) technique was used to describe the tow dimensional picture for the structural changes of the molecule, evaluate the nature of the studied NPs and particle size. Top Keywords Inhibition, TiO2 nanoparticles, Enzyme, Kinetic. Top |