The Activity of 5-Flurouracil Metabolizing Enzyme Dihydropyrimidine Dehydrogenase(DPD) and its Association with Tumor Progression and Markers (CEA, CA19.9) in Patients with Colorectal Cancer AL-Rubaiawi Hadel Kareem1,*, Mohamed Raid J.1 1Department of Chemistry and Biochemistry, Collage of medicine, Al-Nahrain University, Iraq *Corresponding Author: Hadel Kareem AL-Rubaiawi, Department of Chemistry and Biochemistry, Collage of medicine, Al-Nahrain University, Iraq, Email: Hadel_kareem@yahoo.com
Online published on 23 December, 2019. Abstract For the last seventy years, the fluoropyrimidine 5-fluorouracil (5-FU) has been placed as the first line chemotherapy in the treatment of various cancers including colorectal. DPD mediated conversion of 5-Flurouracil to dihydrofluorouracil (DHFU) is the rate-limiting step of 5-Flurouracil catabolism. The response rates of the 5-Flurouracil for advanced CRC is less.5-FU causes severe toxic effects with deficiency of DPD enzyme. The aim of this study was to Evaluate the kinetic and concentration of DPD in tissue with different stage of CRC, as well as Correlation between enzyme concentration and biomarker (CEA.CA19.9). Method By using an ELISA kit for level of DPD and spectrophotometer for activity, in serum and tissue we measured The level and the activity of DPD enzyme in 60 subject in Iraq includes: 30 patients with advance stage of CRC, 30 patients with early CRC and 30 healthy control We also measured serum CEA19.9 and CEA levels. Results The result of the current study showed that serum and tissue activity of DPD in Advance CRC patient very high relative activity compared with early CRC patients and control. Moreover, the activity of DPD in early CRC patient higher than control. Level of tissue DPD shown a significant different between Advance and early CRC patient tissue and normal tissue. While no significant difference in mean serum level of DPD between CRC patient (advance and early) and healthy control. Moreover, DPD showed a moderate correlation with CA19-9 in CRC patients (early and advance) which was close to significant. Conclusions In patients with advanced CRC, there is a hyperactivity of DPD in serum and tumor tissues. Top Keywords Colorectal cancer, Dihydropyrimidin dehydrogenase, 5-Fluorouracil, Elisa. Top |