Compare Transferrin Binding Protein (Tbp A) Gene Virulence of Pasteurella Multocida Type B of Buffalo and Vaccine >Septicemiaepizootica Renata Desty1, Handijatno Didik2, Tyasningsih Wiwiek2 1Master student of Disease Science and Veterinary Public Health, Airlangga University, Jl. Mulyorejo, Kampus C Unair, Surabaya, Indonesia 2Lecture Laboratory of Bacteriology and Micology Department Microbiology, Faculty of Veterinary Medicine, Airlangga University, Jl. Mulyorejo, Kampus C Unair, Surabaya, Indonesia Online published on 4 April, 2020. Abstract Septicemia Epizootica (SE), is a fatal acute septicemia disease by Pasteurella multocida. B: 2, 5 and E: 2, 5 in cattle and buffalo. TbpA has a number of important role for bacterial cells, such as nutrient uptake, transport of molecules in and out of cells and interactions with the environment and host. P. multocida iron acquisition related gene, tbpA, has been considered as an important epidemiological marker. The purpose of this study was to compare the Transferrin Binding Protein A (TbpA) gene from P. multocidatype B of buffalo and it with vaccine strain. Characterization of TbpA of P. multocida and vaccine strain was done using PCR then sequencing. Sample used in this study were P. multocida local isolate from NTT and vaccine strain (Katha). Isolation and identification were performed using bacteriological culture and biochemically characterization. Results from PCR showed positive a tbpA gene on P. multocida local isolate and vaccine strainis. The comparison of the results of sequencing between the tbpa gene isolates P.multocida and vaccines showed deletion. Top Keywords Septisemia Epizootica, Pasteurellamultocida type B, Transferrin Binding Protein A (TbpA) gene, PCR, buffalo. Top |