Sequence Homology and Epitope Prediction of 37 kDa Outer Membrane Protein H(ompH) Gene of Pasteurella Multocida Type B Isolate from Nusa Tenggara Timur (NTT) Maulana Firdausy Kurnia1, Handijatno Didik2,*, Plumeriastuti Hani3, Ernawati Rahaju2, Tyasningsih Wiwiek2, Mufasirin4 1Magister Student on Disease Science and Veterinary Public Health Program, Veterinary Medicine Faculty, Airlangga University, Indonesia, 2Department of Microbiology and Mycology, Veterinary Medicine Faculty, Airlangga University, Indonesia, 3Department of Veterinary Pathology, Veterinary Medicine Faculty, Airlangga University, Indonesia, 4Department of Veterinary Parasitology Veterinary Medicine Faculty, Airlangga University, Indonesia *Corresponding Author: Didik Handijatno, Address: Department of Microbiology and Mycology, Veterinary Medicine Faculty, Airlangga University, Indonesia, Kampus-C, Mulyorejo, Surabaya-60115, Indonesia
Online published on 4 April, 2020. Abstract Haemorrhagic Septicaemia (HS), in Indonesia known as Ngorok disease is a fatal acute septicemia disease in cattle and buffalo caused by Pasteurella multocida. Outer membrane Protein H (OmpH) is one of the major proteins in P. multocida envelope has been purified and characterized as porin immunodominant. The aim of this study is to find out the homology of nucleotide sequence of 37 kDa OmpH gene of P. multocida of local isolate from NTT with vaccine strain (Katha) and 5 referen isolates and predict the epitope of 37 kDa OmpH gene. Detection of 37 kDa OmpH gene was done using PCR. Homology analysis of nucleotide and amino acid sequences was performed on the BLAST program at NCBI. Epitope prediction of 37 kDa OmpH genes P. multocida from NTT and vaccine strains was performed using the online programme B Cell Epitope Prediction Tools. The results showed that 37 kDa OmpH gene of P. multocida from NTT has 99% homology to vaccine strain (Katha) and isolates from China, Iran, India, Mesir, and 89% homology to isolate from USA and 37 kDa OmpH gene of P. multocida local isolate from NTT were likely to be immunogen candidates based on B cell epitope that had 11 epitopes. These findings indicate that P. multocida local isolate from NTT has a chance as immunogen candidate, which can be developed as a vaccine candidate and diagnostic kit for HS. Top Keywords Haemorrhagic septicaemia, P. multocidatype B, outer membrane protein H(omp H) gene, PCR, sequence, epitope. Top |