In Vitro Study of Bronchial Relaxation Mechanism of Coptosapelta Flavescens Korth Root's Methanol Extract On Receptors Kosala Khemasili1,4,*, Widodo Moch. Aris2, Santoso Sanarto3, Soeharto Setyawati2 1Doctoral Program of Medical Sciences, Faculty of Medicine, Brawijaya University, Malang, 2Department of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, 3Department of Microbiology, Faculty of Medicine, Brawijaya University, Malang, 4Department of Pharmacology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia *Corresponding Author: Khemasili Kosala Department of Pharmacology, Faculty of Medicine, Mulawarman University. Jl. Kerayan Kampus Gunung Kelua, Samarinda, 75119, East Kalimantan, Indonesia Tel./Fax. +62-541-747841. e-mail: khemasili_k@yahoo.com
Online published on 4 April, 2020. Abstract Objective To evaluate the bronchorelaxation effect of methanol extract of Coptosapelta flavescens Korth. root (MECFR) on cholinergic, histamine receptors and beta2-adrenoceptors, in vitro. Method The research design was experimental in which a 4-mm long guinea pig's bronchus was kept in an isolated organ bath containing Kreb's-Henseleit solution at 37°C, pH 7.4 and flooded with carbogen gas. The study was divided into 3 groups: in Group 1 and 2, the bronchial rings were incubated in 3 concentrations of MECFR, and then contracted with cumulative doses of methacholine and histamine, respectively; in Group 3, the bronchial ring was incubated in 3 concentrations of propranolol, contracted with a single dose of methacholine, and then the MECFR was administered in cumulative doses. Results In both Group 1 and Group 2, the dose-response curves of all 3 doses of MECFR shifted to the right with lower Emax (P<0.05) as compared to the negative control. In Group 3, the dose-response curves showed MECFR has bronchorelaxation effect on bronchial rings both in the presence of propranolol andwithout, with similar Emax (P>0.05). Conclusion MECFR exhibits bronchorelaxation effects by acting as non-competitive antagonist at the cholinergic and histamine1 receptors, and also by acting as beta2-adrenoceptor agonist. Top Keywords Beta2-adrenoceptor, cholinergicreceptor, Coptosapelta flavescens Korth root, histamine receptor. Top |