Peroxisome Proliferator Activated Receptor (PPAR) Delta Genetic Polymorphism in Saudi Normal Population Almalki Abdulraheem A.1,*, Alsharif Khalaf F.1, Al-Amer Osama M.2, Almalki AbdulAziz A.3, Abdel-Moneim Ahmed S.4,5 1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Al-Taif, Saudi Arabia 2Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, Saudi Arabia 3Department of Medicine, King Faisal hospital, Taif, Saudi Arabia 4Department of Microbiology, College of Medicine, Taif University, Al-Taif, Saudi Arabia 5Virology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt *Corresponding Author: Abdulraheem Almalki, PhD, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif UniversityTaif, Saudi Arabia Phone: 00966127272020 (operator) Ext. 1001 Fax: 00966127273223, Email: abdulraheem.almalki@gmail.com, almalki@tu.edu.sa
Online published on 20 March, 2019. Abstract Background Peroxisome proliferator-activated receptors (PPARs) are transcription factors that belong to the superfamily of nuclear hormone receptors. Three PPARs members, PPAR-alpha, PPAR-delta and PPAR-gamma, have been characterized. PPAR-delta in humans is encoded by the PPARD gene and has been shown to possess a role in regulating fatty acid catabolism and energy homeostasis. Several polymorphisms have been identified in PPARD as potential functional variants. PPARD +294T > C polymorphism was shown to modify serum lipid concentrations in some ethnic groups. Aim The current study aimed to screen the presence of the PPARD polymorphism in the Saudi population. Method Blood samples were collected from normal individuals. Genomic DNA was extracted from peripheral blood monnuclear cells and the partial gene of the PPARD was amplified by polymerase chain reaction and subjected to gel electrophoresis. PPARD rs2016520 polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism. Results The frequencies of CC, CT, and TT genotypes in normal Saudi population were 9.09%, 14.2% and 76.6%, respectively. Conclusion The Saudi nationals seems to have low frequency of the PPARD +294T > C polymorphisms. Limitations Relatively small sample size, serum lipid levels were not determined. Top Keywords Peroxisome proliferator-activated receptors, PPARD and polymorphism. Top |