Prevalence of Panton Valentine Leukocidin (PVL) gene of Staphylococcus aureus Isolated from Different Sepsis Cases in Odisha, India Mohanty Anima1,2, Pal Bibhuti Bhusan2,* 1School of Biotechnology (KSBT), KIIT University-2, 2Microbiology Divisions, ICMR-Regional Medical Research Centre, Chandrasekharpur, Bhubaneswar, India *Corresponding author: Dr. Bibhuti Bhusan Pal, Scientist-F, Senior Deputy Director, Head of Microbiology Division, Address: Microbiology Division, ICMR-Regional Medical Research Centre, Chandrasekharpur, Bhubaneswar-751023, India, Tel: +91-674-2305638, Fax: +91-674-2301351), Email: bbpal_rmrc@yahoo.co.in
Online published on 6 April, 2019. Abstract Panton Valentine Leukocidin (PVL) gene of Staphylococcus aureus is a potent cytotoxin that causes infection in skin and soft tissues which further leads to life threatening diseases. In this study we investigated the prevalence of PVL positivity rate in selected Staphylococcus aureus positive strains isolated from different sepsis cases from Odisha. The samples were inoculated on MacConkey agar, blood agar and nutrient agar plates and significant colonies were tested biochemically. The pathogens were tested against different antibiotics following CLSI guidelines. Polymerase chain reaction (PCR assay) was performed to detect mecA gene and PVL gene using specific primers. Out of 530 strains 425(80.2%) were methicillin resistant S. aureus (MRSA) strains and 105(19.8%) were methicillin sensitive S. aureus (MSSA) strains. PCR analysis revealed that 373(70.4%) were PVL positive MRSA and 51(9.6%) were PVL positive MSSA strains. This study showed high prevalence of PVL gene of Staphylococcus aureus in community acquired infection. Thus it is highly essential to understand the pattern of infection that will help for the treatment and control of community acquired and hospital acquired sepsis caused by S. aureus in this region. Top Keywords Sepsis, Staphylococcus aureus, MRSA, PVL, Odisha. Top |