Association of Certain HLA-II/DR Alleles with Incidence of Thyroiditis in Iraqi Patients Raheem Samir S.1,*, Alkhatib Mussa M.2, Alomari Mohammed M.3 1Department of Microbiology, Collage of Veterinary Medicine-Al-Muthanna University 2Department of Public Health, Collage of Veterinary Medicine-Al-Muthanna University 3Department of Internal and Preventive Medicine, Collage of Veterinary Medicine-Al-Muthanna University *Corresponding author: Samir Sabaa, Department of Microbiology, Collage of Veterinary Medicine-Al-Muthanna University, email: ssrj1964@yahoo.com
Online published on 6 April, 2019. Abstract Background Endocrine epithelial cells, targets of the autoimmune response in thyroid and other organ-specific autoimmune diseases express HLA class II molecules that are presumably involved in the maintenance and regulation of the in-situ autoimmune response. Presents a clear linkage with MHC class II. Moreover, the common auto antigen in either species. A number of studies have shown that either a reduction in the number of T. regs or a reduction in the suppressive abilities of the T.regs may cause self-reactive T cells to remain and lead to autoimmune disease. Human autoimmune thyroid diseases (AITD), such as Hashimoto's thyroiditis and Graves’ disease (GD), are characterized by T and B cell infiltration of the thyroid gland and an intense immune response to well-characterized thyroid auto antigens such as thyroglobulin (Tg). Aim of the study To identify any role for certain alleles in occurrence of the disease. Material and Method Five ml of venous blood samples withdrawn from 30 patients suffering from confirmed thyroiditis disease, 18 patients were females and 12 males in addition to 30 healthy control samples were enrolled in this study all samples were subjected for (ELISA test) (Enzyme Linked Immunosorbent assay) to estimate the TNF-α, and IL-10 Levels by using three ml of blood to extract the serum. Another two ml was used for DNA extraction, and then HLA-Class Il genotyping was performed by polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSO). Results HLA-class II genotyping of thyroiditis patients in comparison with healthy control evoked significant differences in some alleles between both groups. Among DR HLA-DRB1 polymorphism determines susceptibility to autoimmune thyroiditis alleles there were some alleles showed higher frequency in patients group; DR*0403 allele showed increase frequency in patients groups with 35% compared with 6.67% in control group, and the P value was 0.020, which is considered as statistically significant. Another DR*701 allele showed increase frequency in healthy groups with 9 cases 30% and the P value was 0.007. A significant elevation in IL-4 levels and a highly significant increase in IL-10 levels was reported in Thyroidatis patients. Top Keywords Thyroiditis, Genotyping, alleles, IL-4, IL-10. Top |