MicroRNA196a2 rs11614913 Genotypic in Iraqi Newborn Babies with Neural Tube Defects Skakir Alaa Hadi1, Omran Rabab1,* 1Department of Biology, College of Science, University of Babylon, Al-Hillah City, Babel, Iraq *Corresponding auther: Rabab Omran, Professor of Biotechnology-Genetic Engineering in Department of Biology, College of Science, University of Babylon, Al-Hillah City, Babel, Iraq. Email: omranaljelawi@gmail.com.
Online published on 6 April, 2019. Abstract Objective Neural tube defects (NTDs) are complicated human structural deformities occur in newborn babies. The etiology of NTDs is multifactorial contributed both genetic and environmental influences to appear the abnormal phenotype. The analysis of Single Nucleotide Polymorphisms of major contributed genes is still unknown. This paper aimed to investigate miR-196a2 polymorphisms in NTDs newborn patients. The blood samples were collected from newborn babies with NTDs (40 samples) and 40 samples of healthy newborn babies as a control group from August of 2016 to October of 2017. The genotyping of miR-196a2 were performed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. Results There were vital variations within the genotype frequencies of miR-196a2 between patients with NTD and control groups using the P=0.02, OR =0.50 (0.26–0.95). There were significant variations of the genotype TC of patients (27.5%) and healthier (10%) groups and TT genotype. Whereas CC genotype had not any variation between the studied groups. Conclusion Our study suggests that miR-196a2 rs11614913 T > C may contribute to NTD susceptibility but this data required further studies with larger sample size to comprehensive data for confirming our findings and providing a profound conclusion. Top Keywords Tube neural defect, miRNA, rs11614913 SNP, PCR-RFLP. Top |