Protective Effect of Various Lyoprotectant on the Survival of Antineoplastic Drug Producing Serratia Marcescens Kavitha R.1, Damodharan N.2,* 1Associate Professor, Department of Pharmaceutics, SRM College of Pharmacy, SRMIST, Kattankulathur 2Professor, Department of Pharmaceutics, SRM College of Pharmacy, SRMIST, Kattankulathur *Corresponding author: Damodharan N, E-mail: dharan75@gmail.com
Online published on 30 April, 2019. Abstract More effective method of long time storage of the microbes is lyophilisation and cryopreservation. To enable cell survival rate of bacteria during the drying process, lyoprotectant usually added before freezing. Serratia marcescens is a bacterium recognised as the producer of prodigiosin drug which showing promising antibacterial, antifungal, immunosuppressant and anti-proliferative activity in hematopoietic cancer cells. The aim of this current research to investigate the influence of lyoprotectant on the viability of Serratia marcescens during lyophilisation. Several additives were tested as protective agents like sucrose, mannitol, skim milk, bovine serum albumin and sucrose with BSA combination. The effect of drying adjuncts on survival was correlated to their interaction with the bacterial membrane by determination of cell viability by plating and SEM images. Cellular viability during storage of exponential phase cells remained highest for cells dried in the presence of 10%silk milk and 10%sucrose with 5%BSA combination. The result suggested that the possibility of achieving a good formulation system influenced by the type of lyoprotectants used. Top Keywords Lyoprotectant, Serratia marcescens, Mannitol, Bovine serum albumin, SEM analysis. Top |