The Antibacterial Activity of Zinc Oxide Nanoparticles Against Isolates from Internal Cavity of Dental Implant and Natural Teeth (in Vitro Study) Al-Deen Dhyaa M J Hirz1,*, Ali Basima GH2, Al-Mizraqchi Abbas Sabri3 1Department of Periodontics, Master Student, College of Dentistry, University of Baghdad, Iraq 2Department of Periodontics, College of Dentistry, University of Baghdad, Iraq 3Department of Basic Science, College of Dentistry, University of Baghdad, Iraq *Corresponding author: Dhyaa M J Hirz Al-Deen, e-mail: dhaajwad@gmail.com
Online published on 30 April, 2019. Abstract Background Nanotechnology is a new science that has the ability to offer a wide range of uses and improved technologies for biological and biomedical applications. It is considered as the production, categorization, and exploration of materials in the nanometer. This study was aimed to demonstrate the effect of zinc oxide nanoparticles (ZnO NPs) on aerobic and anaerobic bacteria from internal cavity of the dental implants and sulcus of natural teeth in comparison with chlorhexidine 2% and solvent (water 3: 1 ethanol 99.9%.). Materials and Method Aerobic and anaerobic bacteria were isolated from sulcus of natural teeth and internal cavity of dental implants after 3 months from implant placement surgery (flapless design) to 6 female with age range 30 to 44 years with a total of sixteen implants. Different concentrations of ZnO-NPs (6, 2, 1, 0.5, 0.25, and 0.1mg/ml) were prepared. In this in vitro study, disk diffusion method was used to demonstrate the antibacterial activity of different concentrations of zinc oxide nanoparticles to isolated bacteria in comparison to 2% (positive control) and solvent (negative control). Results Aerobic and anaerobic bacterial isolates were sensitive to concentrations (6, 2, 1, 0.5, 0.25, and 0.1mg/ml) of zinc oxide nanoparticles in comparison with CHX and solvent. There was synergistic effect between CHX and 1mg/ml in all agar plates. All concentrations when compared with negative control showed highly significant differences. Conclusion This study shown that zinc oxide nanoparticles were effective against bacteria isolated from internal cavity of dental implant and sulcus of natural teeth. Top Keywords ZnO, Nonoparticles, Aerobic bacteria, Anaerobic bacteria, teeth mplant. Top |