Microbial Analysis of Dento Alveolar bone Immediately after Extraction Muralidharan N. P.1,*, Kritivasan Sahana2, nazar Nabeel3 1Reader, Department of Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University 2Graduate Student, Department of Oral and Maxillofacial Surgery, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University 3Reader, Department of Oral and Maxillofacial Surgery, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University *Corresponding Author: Mr. N.P. Muralidharan Reader, Department of Microbiology, Saveetha Dental College and Hospitals Saveetha Institute of Medical and Technical Sciences, Saveetha University 162, Poonamallee High Road, Chennai-600077, Tamil Nadu, India Phone: +919840560487, Email: mugaidar@yahoo.com
Online published on 4 June, 2019. Abstract Aim This study is done to analyze the microbial load present in the extracted socket immediately after the extraction. Background The oral cavity possesses a varied microbial flora. Extraction of tooth is done primarily due to dental caries. Identification of pathogens in the extraction socket enables us to provide a better medication to post extractions cases. In the previous studies certain bacterial species were isolated as predominant strains which are a part of the normal oral flora. But in recent studies it is found unconventional strains were demonstrated but their role in the pathogenesis is not known. This study is done to explore the other possible strains of the microbes and their frequency of isolation in the extraction site. This will be a pilot study to validate their presence Materials and Method After the extraction procedure was done two samples were collected one sample is collected using swab from the socket and the second sample collected using an excavator. The swab is used for aerobic culture by inoculating in blood agar at 37Oc for 24hrs. The material collected using a sterile excavator is added to sterile cuvettes containing 1 ml Thioglycolate broth and incubated for 48 hrs. After 48hrs the growth were examined by sub culture onto solid media and by smear study. Grams staining was done on the smear and observed under the microscope for bacterial identification. Result In this study there is an increased colonization by many bacterial species in the extraction site. The species isolated are enterococci, micrococci, CONS, lactobacilli, spirochetes, fusobacterium and gram negative cocci. In 3 patients even Candida albicans were detected. Viridans streptococcus was detected in all the samples. Conclusion The study has documented the multiple etiology of the tooth infection and it helps in better understanding of the pathogens present in the extraction socket and to provide a better post extraction care. Top Keywords Anaerobic, aerobic, extraction socket, oral flora, microorganisms. Top |