Histopathological and Molecular Study of Raillietina echinobothrida of Domestic and Wild Pigeons in Al-Muthanna Province Jasim Hussein J.1,*, Abdulzahra Ibrahem A.2, Faraj Katherine B.3, Abed Saif M.4 1Department of Vet. Parasitology, College of Vet. Med. 2Department of Biology, College of Science, University of Al-Muthanna, Iraq 3Department of Microbiology and Vet. Parasitology, College of Vet. Med., University of Basrah, Iraq 4Department of Microbiology science, Samawah, Iraq *Corresponding Author: Hussein J. Jasim, Department of Vet. Parasitology, College of Vet. Med., University of Al-muthanna, Iraq, Email: husseinjasem2014@gmail.com
Online published on 4 June, 2019. Abstract This study was conducted to detect and distinguish Raillietina echinobothrida of domestic and wild infecting pigeons has been based on morphology, histopathological and molecular analysis. The present study was conducted on 115 pigeons (73 of S. Decaocto and 42 of C. livia) of different age groups and of both sexes. The results of the present study were revealed that only 86 out of 115 examined pigeons were infected by Raillietina spp at infection rate of 74.78%. Besides, the percentage of infection with Raillietina spp were recorded in S. decaocto is higher than C. livia in 44.34% and 30.43% respectively. The most isolated parasites of examined birds were showed the cestoda R. echinobothrida with total number (168) adult worms from total number of examined pigeons infected (115). The histopathological examination of small intestine of pigeons infected with R. echinobothrida were showed different changes like, an infiltration of inflammatory cells in lamina properia and atrophy of villa of small intestine with vaculation of muscularis externain and loss of mucous membrane. The result of PCR were showed that specific primers (3S-F, BD2-R) were successfully for amplifying 944 bp with flanking region of ITS2 to R. echinobothrida. Also, the PCR result were revealed that 61(70.93%) out of 86 birds were positive to R. echinobothrida. Top Keywords R.echinobothrida, domestic and wild pigeons, Pathological, Molecular, Iraq. Top |