The Ameliorative Effects of chrysin against Methandienone-Induced change in blood Parameters in Mice Falih Marwah Y.1,*, Mohammed Abbas A.1, Sulaiman Ghassan M.1 1Biotechnology Division Applied Science Department, University of Technology, Baghdad, Iraq *Corresponding Author: Marwah Y. Falih, Biotechnology Division, Applied Science Department, University of Technology, Baghdad, Iraq, Phone: +964 (0) 7704449837, Email: xxmarwa87xx@yahoo.com
Online published on 4 June, 2019. Abstract Objective investigate the effects of methandienone on blood cells and compared to those of normal and chrysin supplementation conditions Materials and Method Male Mus musculus mice (8–12 weeks in age, 25.00 ± 2.00 gm weight) were acquired, seven experimental groups, each with 5 mice. The negative control group was treated with distilled water. The positive control group was treated with 20mg/kg of cyclophosphamide (CFA) twice a week for two weeks. Methandienone-treated group received the drug (1mg/kg body weight) orally on a daily volume of 0.1 ml for one week. The first chrysin-treated group received the drug (25mg/kg body weight) orally on a daily volume of 0.1 ml for one week. The second chrysin-treated group received the drug (50mg/kg body weight) orally on a daily volume of 0.1 ml for one week. The 6th group received 1mg/kg methandienone received an additional oral treatment with chrysin (25mg/kg) for one week. The 7th group received 1mg/kg methandienone received an additional oral treatment with chrysin (50mg/kg). Results The results showed clear changes in blood parameters in treated animals, represented by significant reduction in the counts of total leucocyte, monocyte, eosinophil and neutrophil, associated by significant increase in the counts of lymphocyte, red blood cells, and blood counts, as well as hemoglobin level. Conclusion Methandienone causes change in blood parameters and chrysin play an ameliorative effect of methandienone. Additionally, treatment with chrysin for short period was able to reduce these side effects of treatment with methandienone. Top Keywords Methandienone, chrysin, cyclophosphamide, complete blood tests, mice. Top |