Stemness of Pulp Cells From Supplemental Tooth During the Extended Culture Shin Jisun1, Kim Jongbin1, Shin Seungwoo2, Shim Younsoo3, Soyoun An4, Ahn Eunsuk5, Kim Jongsoo6 1Assistant Professor, Department of Pediatric Dentistry, Dankook University, Korea 2Fellow, Department of Pediatric Dentistry, Dankook University, Korea 3Associate Professor, Department of Dental Hygiene, Sunmoon University, Korea 4Assistant Professor, Department of Pediatric Dentistry, Wonkwang University, Korea 5Assistant Professor, Dept. of Dental Hygiene, Kyungbok University, Korea 6Professor, Department of Pediatric Dentistry, Dankook University, Korea Online published on 4 June, 2019. Abstract Background/Objectives The aim of this study was to evaluate the potential of stemness of the human dental pulp cells from supplemental tooth during the extended culture. Methods/Statistical analysis Supplemental tooth was collected from a patient who was 5-year-old and soon after extraction of the tooth, the primary cells were cultured from the pulp tissues. Those were subcultured until 16th passage and each passage cells were evaluated their stemness with expression of cell surface markers by using flow cytometric analysis. Findings The cell surface markers which are representative mesenchymal stem cell markers, such as CD44, CD73, CD90 and CD146 were expressed during the whole passages. The expression of these markers showed second peak at 6–7 passages and slightly decreased after 8th passage during the extended culture of dental pulp cells from this supplemental tooth. In addition, those cells were confirmed with negative expressions of CD24, CD146 markers. The proliferation rate of stem cells from the pulp of supplemental tooth was two to three times fast comparing to the stem cells from other dental pulp tissue. Improvements/Applications Pulp tissue in supplemental tooth could be a choice for donor site of stem cells since it was confirmed that they possessed stemness, especially strong in early passages. Top Keywords Stem cells, Supernumerary tooth, Supplemental tooth, Flow cytometry, Extended culture. Top |