A Comparative between Virus Type 1 Original Sabin Polio Virus and Sabin AFP Case in Cytopathic Effect Characteristic Al-Garawi Razaq Hadi Eissa1,*, Alarajy Kadhim Hashim2, Zgair Ayaid Khadem3 1Department of Virology, The National polio Laboratory (NPL), Central Public Health Laboratory (CPHL), Ministry of Health (MOH), Baghdad-Al-Andlus Street, Iraq 2Department of Biology, College of Science, Mustansiriyah University, Iraq 3Department of Biology, College of Science, University of Baghdad, Iraq, Baghdad-Al-Jadiria, Iraq *Corresponding author: Razaq Hadi Eissa Al-Garawi, E-mail: razaqhadi70@gmail.com
Online published on 4 June, 2019. Abstract Acute flaccid paralysis (AFP) is a clinical syndrome characterized by rapid onset of weakness, affecting the muscles then progressing to maximum severity within several days to weeks and sometimes may lead to death. National Polio Laboratory (NPL\ IRAQ) is the reference laboratory that is responsible for the laboratory part of AFP surveillance project and A total of two hundred and fifty (250) stool samples were collected during the period (March to November 2017 from all Iraqi provinces. The main aimed of this study was isolating and differentiate of poliovirus strains type 1 AFP (Sabin virus) from the stool samples of children received polio vaccine and suffering with acute flaccid paralysis. Then comparative with original strains of sabin polio vaccine type 1 in cytopathic effect characteristic. CPE is the visible result of viral infection. Current study shows For each type of polio infections have specific phenotypical CPEs on cell culture. When compare viruses’ CPEs characteristic between sabin polio vius AFP strain 1 and original Sabin polio virus strain 1 there are more differ occurred after inoculation on cell culture. CPE rate with sabin polio vius AFP strain 1 consider at high multiplicity of infection MOI and all CPEs occur rapidly with increase in cell morbidity rates because it have severe type of CPE called Total destruction CPE.while the study show that CPE of original Sabin strain type 1 are weakly. Top Keywords Cytopathic effect characteristic, sabin AFP case, virus type 1, original sabin polio virus, and National Polio Laboratory. Top |