Impact of Anesthesia on Adaptive Immune Response in Orthopedic Surgery Aubaid Adnan Hamad1, Yaseen Khalid Lahmood2,* 1Professor, Department of Medical Microbiology, College of Medicine, University of Al-Qadisiyah, Diwaniyah, Iraq 2Ph. D Student at Department of Medical Microbiology, College of Medicine, University of Al-Qadisiyah, Diwaniyah, Iraq *Corresponding Author: Khalid Lahmood Yaseen, Email: yaseenkhalid203@gmail.com
Online published on 4 June, 2019. Abstract Background Anesthesia is thought to be main factor affecting patients’ postoperative outcomes. Current study was carried out to evaluate the possible role of anesthetics and surgery on cellular immunity by measuring serum levels of IL-2 and IL-10 as well as CD4 and CD8 lymphocytes counts. Materials and Method Serum levels of IL-2 and IL-10 cytokines were measured by using enzyme linked immunosorbent assay (ELISA) technique. Flow cytometry was used to determine CD4 and CD8. Comparisons for statistical significance were performed using Mann-Whitney U test. Result Observation of CD4 lymphocytes counts reveled significant rise during time of anesthesia (42.23pg/ml), however they showed significant reduction post-operatively (37.12pg/ml), but the reduction did not reach baseline count. In addition, the observation of CD8 lymphocytes counts reveled significant rise during time of anesthesia (28.21pg/ml), however it showed significant reduction post-operatively (22.91pg/ml), but the reduction did not reach baseline count. Serum levels of cytokine IL-2 were higher post-operative (1257.7pg/ml), and lower levels were seen with IL-10 mainly pre-operative (36.08pg/ml). However, levels of interleukin-2 and interleukin-10 showed no significant changes in relation to time of anesthesia whether pre-, peri-or post-operatively (P>0.05). Conclusion There was no significant association between cytokines levels, IL-2 and IL-10, or CD4 and CD8 counts with type of anesthesia whether general, local or regional anesthesia (P > 0.05). Top Keywords Anesthesia, adaptive immunity, IL-2, IL-10, CD4, CD8, flow cytometry, ELISA. Top |