Detection of EML4-ALK Fusion Gene Rearrangement in Non-Small Cell Lung Carcinoma in a Sample of Iraqi Patients: A Comparison of Chromogenic in Situ Hybridization with Correlation of ALK Protein Expression Farhood Rawaa Ghalib1,*, Al-tariahi Kaswer2, Adul-Sahieb Rehab Hameed2, Mamoori Afraa1 1Department of Pathology, College of Medicine, University of Babylon, Babylon, Iraq 2Department of Pathology, College of medicine, University of Kufa, Najaf, Iraq *Corresponding author: Rawaa Ghalib Farhood, E-mail: dr.rawaaghali123@gmail.com
Online published on 4 June, 2019. Abstract Background Lung cancer is a major public health problem. It is the most common cause of cancer-related death in men and the second most common in women. Moreover, EML4-ALK(the echinoderm microtubule-associated protein like4-anaplastic lymphoma kinase fusion gene) has been identified as a potent oncogenic driver in non-small-cell lung carcinoma (NSCLC), in particular adenocarcinoma (ADC) and less commonly squamous cell carcinoma. The aim of current of study was to investigate ALK protein overexpression and its correlation with EML4-ALK gene rearrangement by CISH. Method The paraffin embedded blocks were retrospectively collected from 80 patients diagnosed with NSCLC. ALK protein study was performed by using Monoclonal Mouse Anti-Human for IHC and for EML4-ALK fusion gene by using CISH (CISH working solution/Zytovision, Germany). Results ALK protein overexpression was detected in (10%, n= 8/80) of NSCLC and the ALK gene rearrangement was (7.5%, n= 6/80). There was high concordance in the assessment of ALK gene rearrangement by CISH techniques and ALK protein expression by IHC (P= 0, r= 0.94). Conclusion IHC and CISH were very well correlated in detection of ALK protein expression and ALK gene rearrangement, respectively. Also, CISH is a new and highly practical method to detect EML4-ALK, therefore, it could be introduced in plans of ADC management and less commonly SCC of the lung. Top Keywords Lung cancer, EML4-ALK, NSCLC, IHC, CISH, ALK, TNM. Top |