Isolation and Identification of Prevotella Intermedia by Culture and Polymerase Chain Reaction Mahdi Aula Abdullah1,*, Al Warid Rasha Jasim2, Al-Ameedee Ameer Hamdi3 1B. Sc. Veterinary Medicine and Surgery 2Medical Microbiology Department 3Operative Dentistry Department, College of Dentistry, Babylon University, Babylon Province *Corresponding author: Aula Abdullah Mahdi, E-mail: Draulla 201993@gmail.com
Online published on 4 June, 2019. Abstract Background Endodontic disease is the result of both the pathogenic effects of the microbes and response of the host. The most common species isolated from primary endodontic infections are black pigmented bacteria. These bacteria are obligate anaerobes, non-motile and non-spore forming, which are oxygen sensitive organisms. These organisms are implicated in apical abscess formation due to their proteolytic activity. Prevotella species such as Pr. intermedia have been cultured from 26 ‒40% of infected root canals, suggesting an association with proteolytic activity the isolated bacteria from clinical samples are difficult in growth supplies, it may give flexible effects with obtainable biochemical test, so it is not all the time detected or might be misidentified during the detection. The 16SrRNA gene has brought an original instrument for approximating bacterial phylogeny. The Aim of this study was to investigate the presence of Black-Pigmented Bacteria, Pr. Intermedia in Endodontic Infections By Culture Method And Polymerase Chain Reaction (PCR) Analyses; Also Detection Biochemical, Virulence Potential of microbial isolate and examine the antibiotic sensitivity test to isolated bacteria. Methods The study population contained of thirteen patients, ages ranged from nineteen to fifty five years awarding at the endodontic unit in the Dentistry College of the University of Babylon. Ten isolates (33.3%) of Pr. intermedia were reported as positive by 16SrRNA gene whereas only six isolates (20%) reported positive results by traditional culture and biochemical test. Results All isolates showed positive results for several virulence factors. Of the selection six antibacterial drugs evaluated against all the Bacterial isolate: amoxicillin, Augmentin and penicillin G were highly effective in terms of maximum diameter of growth inhibition zones. Clindamycin and Erythromycin were found to be moderately effective against the isolated bacteria. Metronidazole was not effective against the bacteria as it did not show any inhibitory activity. Conclusions The presence of Pr. intermedia suggests that a significant role is played by these organisms in the pathogenesis of Endodontic infections. Furthermore, PCR provided more frequently detected endodontic Black pigmented bacteria than did culture. All isolates capable to produce biofilm, gingpine and ability to adherence. Based on the study results, amoxicillin-clavulanic acid and amoxicillin were the most potent antibiotics most antimicrobial agents in vitro, against Pr. intermedia isolated from root canal infection, therefore can be used treatment root canal infection. Top Keywords Endodontic disease, Pr.intermedia, 16S RNA gene, PCR, biofilm. Top |