The Role of Frizzled-Related Protein3 Frzb3 Gene Polymorphisms` in Iraqi Patients with Knee Osteoarthritis Hassan Nadia N.1,*, Alwasiti Estabraq A.2, Jassim Nizar Abdulateef3 1Ministry of Health, Directorate of Technical Matters, Lab. Departments 2Dept. of Chemistry and Biochemistry, College of Medicine, Al-Nahrain University, Iraq 3Rheumatology Unit Dept. of Medicine, College of Medicine, University of Baghdad, Iraq *Corresponding author: Nadia N. Hassan, E-mail: nadianoori114@gmail
Online published on 4 June, 2019. Abstract Osteoarthritis is a degenerative articular disease that has a complex pathogeny because various risk factors interact in the process of cartilage deterioration. From the 50’s it´s known that certain forms of osteoarthritis are related to an alteration of genetic component. This study aim to investigate possible association between KL grade, clinical features (WOMAC) and susceptibility polymorphisms to OA, such as FRZB3 gene, to better define the grading of this disease for prevention, early diagnosis, and treatment. Total of 120 Iraqi patients affected by primary OA, aged (45 years and above) and 60 healthy people (control) at the same age range, with no family history of OA were evaluated at Al-Imamein Al-Kadhimein Medical City (Rheum. Dept.). The degree of severity of knee OA was assessed by clinical and radiographic assessment. FRZB rs7775 genotyping is performed using DNA sequencing and the level of serum sfrp3 protein is measured using Human sfrp3 ELISA Kit Genotyping of FRZBrs7775 polymorphisms revealed that three different genotypes. Also it was revealed highly significant difference in the two alleles (C, G) frequencies between control and Knee OA patients (p> 0.001) that the highest allele G frequency was found in Knee OA patients group (odd ratio 13.1) with CI (6.12–28.03). No significant difference was noted in the serum concentration of sFRP3 between the three groups (severe, mild and control). In this study, 120 of Iraqi people with severe and mild Knee OA are mostly of GG and CG genotypes in the FRZB3rs7775 gene polymorphisms and this alteration in genotype of FRZB3 gene doesn't affect the serum level of sFRP3 protein. Top Keywords Frizzled-related protein3 FRZB3 gene, secreted frizzled-related protein3 sfrp3. Knee osteoarthritis. Top |