Effect of Adding Probiotic with Different Levels of Citric Acid in Feed Supplementation to Laying Hens on Bacteria Content of Feed, Litter and Egg Shell Al-Dalawi Rashid H. Hameed1, Ameen Qana Hussein1, Jasim Mahdi Salih1 1College of Agriculture, University of Kirkuk, Iraq 2College of Agriculture, University of Dayala, Iraq Online published on 19 August, 2019. Abstract Study conducted to determined effect of adding Probiotic (Iraqi Probiotic) with different levels of citric acid to Lohmann brown laying hens in order to know the effect of addition on the microbial content of the feed, litter and eggshell. The treatments were T1 without any addition (control), T2 (0.25) probiotic, T3, T4 and T5 add Probiotic each with a fixed level of 0.25% with levels 0.15, 0.30, 0.45% of citric acid sequentially. The Addition of probiotic with citric acid led to a significant decrease (p <0.05) in both (Total count bacteria) and (Coliform bacteria) in feed and litter compared with the control treatment and the treatment of adding probiotic alone. The addition of either alone or with citric acid lead to a significant decrease in the total number of aerobic bacteria on the eggs product and no effect on the number of E.coli in the eggs product. Top Keywords Probiotic, citric acid, laying hens, feed, eggs shell, litter, bacteria Count. Top |