Comparing Lower Grades and Higher Grades Astrocytoma in Three Age Groups: by CD133 Antibody Saadoon Zainab Zahid1,*, Al-Khateeb Huda Mahdi1, Alkhafaji Khitam Abdul-Razak1 1College of Medicine, University of Baghdad, Iraq-Baghdad *Corresponding Author: Zainab Zahid Saadoon, College of Medicine, University of Baghdad; Iraq, Baghdad, Postal code 00964, Phone: 0700254259 Email: zainab.z@comed.uobaghdad.edu.iq
Online published on 19 August, 2019. Abstract Brain tumors are heterogeneous in nature and current studies reveal that aggressive behavior and recurrence of the tumor are led by brain tumor stem cells (BTSCs) This stem cells compartment demands distinct study to provide new therapeutics lines targeted against it. From the many markers that annotate BTSCs, is CD133, the first marker used to identify BTSCs and regarded as the protein which confers resistance and virulence of the tumor. Aim of Study Using CD133, to identify BTSCs by immunohistochemistry in paraffin sections of different grades of astrocytoma, distributed in to three different age groups then, compared with each other. We aimed to check the change in number of CD133-positive cells between grades and between ages, to confirm that CD133 is a real representative for the actual increase in virulence across grades and ages. Materials and Methods 121 paraffin blocks are collected from the archives of specialty surgery hospital in Baghdad and processed for immunostaining with CD133, then quantified to calculate number of positives and comparing the results between different grades and different ages. Results We obtained increased in number of positives of CD133 by ascending from lower grades towards higher grades in the three age groups; however, when we compared the same grade in between the 3 groups, we saw there was increase in expression from the children to the adult and then decrease in the older, the adulthood show the most increase. The ascending manner of the expression is no longer demonstrable across the age groups, that's the older group show lower expression than the adults despite being more virulent clinically. This mean that CD133 doesn't stand alone as a candidate for representation of the virulence in age group ≥50 years old. Top Keywords Astrocytoma, grades, ages, CD133, virulence. Top |