Il-18-137G/c (rs187238) Gene Polymorphisms in Pulmonary Tuberculosis iraqi Arab Patients Utba Nawal Mohammed* Department of Biology, College of Science, University of Baghdad, Al-Jaderia, Baghdad, Iraq *Corresponding Author: Nawal Mohammed Utba, Department of Biology, College of Science, University of Baghdad, Al-Jaderia, Baghdad-10071, Iraq, Phone: +964 7901826834, Email: anooutba2014@yahoo.com
Online published on 13 November, 2019. Abstract Tuberculosis (TB) primarily caused by Mycobacterium tuberculosis and remains a worldwide problem despite well documented, well publicised methods of prevention and cure. 80 pulmonary tuberculosis (PTB) patients without HIV infection were included in this study. Patients were divided into three groups; 40 MDR (multidrug resistant), 20 RD (Recently diagnosed) and 20 OC (Old cases). While the others 89 apparently healthy subjects (control group, (HC)). IL-18 serum level was assessed by using ELISA technique. However, polymorphism was analyzed using polymerase chain reaction-single specific primer (PCR-SSP), at the position-137 G\C (rs187238) in the promoter of IL18 gene. The median serum level of IL-18 for all patient groups was significantly higher than HC group. Comparing PTB patient groups to HC group revealed that none of genotypes or alleles showed significant differences between patients groups and HC group. The results are indicated that IL-18-137 alleles and genotypes showed no association with the risk of PTB development in Iraqi Arab population or protection against it. Top Keywords Pulmonary tuberculosis, Multidrug resistance drug, IL18 gene polymorphism, ELISA, Iraq. Top |