Using Random Amplified Polymorphic DNA (RAPD) Fingerprinting Technique to Analyze Genetic Variation in Staphylococcus Aureus Isolated from Different Sources in Babylon Province Hospitals Banoon Shaima R.1,*, Kadhim Zahraa Kamil2, Aziz Zahid S.1, Jameel Zahraa isam3, EWadh Ruqaya M.J.4 1Biology Department, College of Science, University of Misan, Maysan, Iraq 2Biology Department, College of Science, University of Kufa, Najaf, Iraq 3The Islamic University, Babylon, Iraq 4College of Pharmacy, University of Babylon>, Babylon, Iraq *Corresponding Author: Shaima R. Banoon, Biology Department, College of Science, University of Misan, Maysan, Iraq, Email: shimarb@uomisan.edu.iq
Online published on 13 November, 2019. Abstract Genetic fingerprinting of 19 different isolates of Staphylococcus aureus from wounds, skin, nails and urinary tract infection taken from patients who admitted at AL-Hilla General Teaching Hospital and Babylon Hospital for Maternity and Pediatric, using random amplified polymorphic DNA (RAPD) was carried out. Two (OPY07 and OPX-20) primers showed polymorphism among the isolates tested generating 12 bands, 8 of which were polymorphic with sizes ranging between 250 and 1 kb. All the isolates were classified completely into one major group with nineteen different subgroups. The nineteen different subgroups suggest adaptation of S. aureus in the different host cells. This indicates possible relationship between host origin and genetic variation among S. aureus isolates. The DNA fingerprint defined for each strain of S. aureus could be useful in epidemiological studies, medical diagnosis and the identification of new strains and their origins. Top Keywords Staphylococcus aureus, RAPD, PCR, Fingerprinting Technique, OPY-07 and OPX-20. Top |