Effect of low methionine formula on levels of IL-1β Serum and IL-1β gene expression in knee joint cartilage tissues of normal rabbits and ACl induction OA models Sutjiati Endang1, Kusworini2, Wirjatmadi Bambang3, Kalim Handono4 1Doctoral Students of Postgraduate Program of Medical Sciences, Faculty of Medicine, Department of Nutrition, Brawijaya University Health Polytechnic of Malang, Indonesia 2Department of Clinical Pathology, Faculty of Medicine, Brawijaya University, Indonesia 3Department of Nutrition, Faculty of Public Health, Airlangga University, Indonesia 4Department of Internal Medicine, Faculty of Medicine, Brawijaya University, Indonesia Online published on 1 November, 2018. Abstract Methionine deficiency is an environmental factor can degrade the quality of the bone, cartilage and modulate chondrocytes to increase protease secretion and change gene expression. The aim of the study was to determine the effect of methionine deficiency on IL-1β serum, expression of IL-1β in cartilage tissue of knee joints of New Zealand rabbit's (Oryctologus cuniculus). The experimental animals were divided into 6 treatment groups: normal group with the addition of DL-methionine 0.25%, normal group with the addition of DL-methionine 0.15%, normal group with the addition of DL-methionine 0.00%, ACL group with the addition of DL-methionine 0.25%, ACL group with the addition of DL-methionine 0.15%, ACL group with an addition of 0.0%. Examination of IL-1β serum by ELISA with RayBio Rabbit commercial kit. Examination of IL-1β expression immunohistochemically using IL-1β anti-rabbit primary antibody with a Santa Cruz commercial kit (Sc7884). The results were analysed using one way ANOVA, followed by LSD. The results indicate that methionine deficiency (DL-methionine 0.0%) is able to increase IL-1β serum. The expression of IL-1β in knee joint cartilage tissue appears to be increased significantly through the metabolic effects or interactions with biomechanical changes. Methionine deficiency has the same ability in normal and pathological conditions, has a tendency to increase IL-1β serum and IL-1β gene expression in joint cartilage tissue. Top Keywords Methionine deficiency, Il-1β serum, IL-1β expression, Knee joints cartilage. Top |