The effects lactobacillus crispatus probiotics on proliferation and metastasis of cervical cancer cell line using 3d cell culture Adnan Azher1,3, Motevaseli Elahe2, Sadroddiny Esmaeil1 1Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran 2Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran 3International Campus, Tehran University of Medical Sciences, Tehran, Iran Online published on 9 January, 2019. Abstract Objective Probiotics has been tried as an adjuvant therapy for malignant tumors through their abilities to modulate intestinal microbiota, host immune response and gene expression. Further, there are emerging data supporting the role of probiotic lactic acid bacteria in prevention of early stages colon cancer development but data on their effect on advanced colorectal cancer and cervical cancer are limited. Therefore, the aim of this study was to evaluate the impact of lactobacillus crispatus supernatant (LCS) on the human cervical cell lines (HeLa) proliferation and metastasis. Materials and Method Effects of Lactobacillus supernatant on cell invasion in vitro was assessed by Transwell migration and invasion assays (3-D culture). The cytotoxic activity of Lactobacillus crispatus supernatant were determined by MTT assay and compared to controls, De Man Rogosa Sharpe (MRS) media and MRS + lactic acid groups. Expression of Matrix Metalloproteinase-2 (MMP2) and-9 (MMP9) genes was performed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) following the cell synchronization. Results Lactobacillus crispatus supernatant had cytotoxic and anti-metastatic effect on HeLa but not on normal cells. Down-regulation of MMP2 and MMP9 genes expression was also observed post-LCS treatment as compared to controls, De Man Rogosa Sharpe (MRS) media and MRS + lactic acid groups (P < 0.05). Conclusion Lactobacillus crispatus Supernatant could be a potential therapeutic agent for the treatment of advanced human cervical cancer through cytotoxic and anti-metastatic mechanisms Top Keywords Cervical cancer, Lactobacillus crispatus supernatant, Cell Line, Metastasis. Top |