Establishment of a Breast Cancer Biobank for Translational Research: A Single Institutional Pilot Study Nare Smeeta1, Patil Tanvi2, Dixit Santosh3, Jere Pooja4, Verghese Beenu5, Krishnan Lakshmi6, Busheri Laleh7, Deshmukh Chetan8, Koppiker Chaitanyanand9 1Clinical Researcher, Orchids Breast Health Clinic, Prashanti Cancer Care Mission, Senapati Bapat Road, Pune, India 2Student, Orchids Breast Health Clinic, Prashanti Cancer Care Mission, Senapati Bapat Road, Pune, India 3Clinical Research Scientist, Orchids Breast Health Clinic, Prashanti Cancer Care Mission, Senapati Bapat Road, Pune, India 4Radiologist, Orchids Breast Health Clinic, Prashanti Cancer Care Mission, Senapati Bapat Road, Pune, India 5Sr. Radiologist, Orchids Breast Health Clinic, Prashanti Cancer Care Mission, Senapati Bapat Road, Pune, India 6Research Scientist, Orchids Breast Health Clinic, Prashanti Cancer Care Mission, Senapati Bapat Road, Pune, India 7Research Coordinator, Orchids Breast Health Clinic, Prashanti Cancer Care Mission, Senapati Bapat Road, Pune, India 8Medical Oncologist, Orchids Breast Health Clinic, Prashanti Cancer Care Mission, Senapati Bapat Road, Pune, India 9Physician, Orchids Breast Health Clinic, Prashanti Cancer Care Mission, Senapati Bapat Road, Pune, India Online published on 16 March, 2018. Abstract Introduction/Background Current Breast Cancer (BC) knowledge has primarily emerged from translational research in western populations. Given the genomic and population diversity, mere extrapolation of this knowledge to BC in Indian women is incorrect. Hence, Indian-population specific research resources are needed1. Herein, we describe the creation of a pilot BC BioBank as a repository of clinically annotated biospecimens from BC patients at our clinic. Purpose to establish a BC Biobank for collaborative translational research Methodology Given its high prevalence in India, our pilot study focuses on a BC subtype named triple Negative BC (TNBC). Retrospective data (i.e., medical history, clinico-pathology, imaging, surgery, chemotherapy and post-treatment status etc.) was collected after informed consent from TNBC patients. Formalin-fixed paraffin-embedded (FFPE) tissue from post-surgery samples was also collected. In addition, Radiology images of TNBC were analyzed to identify unique imaging characteristics. Results and Discussion Out of 115 registered TNBC cases at our clinic, a pilot BioBank was generated with clinical data and FFPE blocks of 44 samples. Clinico-pathology details and medical history were meticulously collected for this study cohort. From these cases, extensive radiology imaging analysis was performed on 25 cases to identify unique Mammography or SonoMammography features of TNBC in Indian women. Research Implications The DNA from FFPE blocks of TNBC tumors is being used to identify mutational patterns in cancer-specific genes. The TNBC Imaging database has generated collaborations with imaging scientists to identify unique radiological features of Indian TNBCs. Novelty/Originality In this project, we have created a novel research repository for TNBC-themed research in Indian women. this unique project is acting as a driver for multidisciplinary research collaborations with scientists from academic and private sectors. Top Keywords Biobank, Triple Negative Breast Cancer, Translational Research. Top |