Analysis of Methanolic Extract of Fusarium Chlamydosporum using GC-MS Technique and Evaluation of its Antimicrobial Activity Mohammed Ghaidaa Jihadi1, Hameed Imad Hadi2,*, Kamal Sabreen A.3 1Department of Biology, College of Science, University of Al-Qadisiyah, Hillah city, Iraq 2Biomedical Science Department, University of Babylon, College of Nursing, Hillah city, Iraq 3Department of Biology, College of Science for women, University of Babylon, Hillah city, Iraq *Corresponding Author: Imad Hadi Hameed Biomedical Science Department, University of Babylon, College of Nursing, Hillah city, Iraq; Phone: 009647716150716; E-mail: imad_dna@yahoo.com
Online published on 3 April, 2018. Abstract Chromatography is the term used to describe a separation technique in which a mobile phase carrying a mixture is caused to move in contact with a selectively absorbent stationary phase. The objectives of this study were analysis of the secondary metabolite products and evaluation antimicrobial activity. Bioactives are chemical compounds often referred to as secondary metabolites. Eleven bioactive compounds were identified in the methanolic extract of Fusarium chlamydosporum. DL-Arabinose, D-Glucose, 6-O-α-D-galactopyranosyl, α-D-Glucopyranoside, O-α-D-glucopyranosyl, 5-Hydroxymethylfurfural, N-(4, 6 Dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylidenea, 1H-Purin-2-amine, 6-methoxy-N-methyl, 2-Methyl 9-β-d-ribofuranosylhypoxanthine, 1-Hexadecanesulfonic acid, 3, 5,-dichloro-2, 6-dimeth, Methyl-6, 7 benzoisoquinoline, Undeca-2, 4, 6, 8, 10-pentaenal, 11-(2-furyl)-, oxime, and 2-Bromotetradecanoic acid. Malva sylvestris was very highly active 6.85±0.25 mm. The results of anti-fungal and anti-bacterial activity produced by Fusarium chlamydosporum showed that the volatile compounds were highly effective to suppress the growth of Aspergillus terreus (6.09±0.21) and Staphylococcus aureus (5.99±0.19) mm. Top Keywords Anti-Microbial, Fusarium chlamydosporum, GC-MS, Secondary metabolites. Top |