Characterization of Antimicrobial Metabolites Produced by Salvadora persica and Analysis of Its Chemical Compounds using GC-MS and FTIR Hameed Rafid Hadi1, Mohammed Ghaidaa Jihadi2, Hameed Imad Hadi3,* 1Ministry of Health, Mesan Health office, Mesan city, Iraq 2Department of Biology, College of Science, University of Al-Qadisiyah, Hillah city, Iraq 3Biomedical Science Department, University of Babylon, College of Nursing, Hillah city, Iraq *Corresponding Author: Imad Hadi Hameed Biomedical Science Department, University of Babylon, College of Nursing, Hillah city, Iraq; Phone: 009647716150716 E-mail: imad_dna@yahoo.com
Online published on 3 April, 2018. Abstract Meswak (Salvadora persica) is one of the most commonly used medicinal plants for oral hygiene among global Muslim community. The objectives of this study were analysis of the secondary metabolite products using GC-MS and FTIR and evaluation of antimicrobial activity. The FTIR analysis proved the presence of alkyl halides, alkanes, and alkenes which shows major peaks at 960.55, 1029.99, 1097.50, 1141.86, 1321.24, 1373.32, 1616.35, 1723.65, 2852.72 and 2922.16. Our research recorded maximum zone formation was against Staphylococcus aureus (6.635±0.25). Salvadora persica was very highly active against Aspergillus terreus 6.77±0.24. Twenty one bioactive compounds were identified in the methanolic extract of Salvadora persica. GC-MS analysis of Salvadora persica revealed the existence of the 5α-Androstan-16-one, cyclic ethylene mercaptole, Glycerin, 3, 5-Dithiahexanol 5, 5-dioxide, 1H-Pyrazole-1-carbothioamide, 3, 5-dimethyl-, 17-Octadecynoic acid, Benzenepropanoic acid, α-(hydroxyimino)-, Indan-1, 2-dione, 4-methyl-, Butanoic acid, 4, 4’-dithiobis[2-amino-, [S-(R*, R*)]-, 5-Hydroxymethylfurfural, 1H-Pyrrole, 1-(phenylmethyl)-, Cyclopentanone, 2-cyclopentylidene-, Phenol, 2-methoxy-6-(1-propenyl)-, 2, 7-Diphenyl-1, 6-dioxopyridazino[4, 5: 2’, 3’]pyrrolo[4’, 5’-d]pyrida, 2-(4-(But-2-yl)phenyl)propanoic acid, 3H-Cyclodeca[b]furan-2-one, 4, 9-dihydroxy-6-methyl-3, 10-dim, Phenol, 2, 6-dimethoxy-4-(2-propenyl)-, Butylaldehyde, 4-benzyloxy-4-[2, 2,-dimethyl-4-dioxolanyl]-, Benadryl, 9-Hexadecenoic acid, i-Propyl 11, 12-methylene-octadecanoate and 2, 6-Bis[2-[2-S-thiosulfuroethylamino] ethoxy]pyrazine. Top Keywords GC-MS, FT-IR, Salvadora persica, Pseudomonas aerogenosa, Proteus mirabilis, Staph. aureus, Klebsiella pneumonia. Top |