In Vitro Anti-diabetic Properties of Methanolic Extract of Thymus vulgaris Using α-glucosidase and α-amylase Inhibition Assay and Determination of its Bioactive Chemical Compounds Aljarah Ali Kadhim1, Hameed Imad Hadi2,* 1Department of Biology, College of Science for women, University of Babylon, Hillah City, Iraq 2Biomedical Science Department, University of Babylon, College of Nursing, Hillah City, Iraq *Corresponding author: Imad Hadi Hameed. Biomedical Science Department, University of Babylon, College of Nursing, Hillah City, Iraq; Phone number: 009647716150716; E-mail: imad_dna@yahoo.com
Online published on 3 April, 2018. Abstract The objectives of our research were analysis of the bioactive chemical metabolite products and determination In vitro anti-diabetic properties. The results of anti-diabetic activity produced by Thymus vulgaris showed that the volatile compounds were effective to α-glucosidase and α-amylase inhibition. The purification of compounds produced by Thymus vulgaris can be useful. Twenty seven bioactive compounds were identified in the methanolic extract of Thymus vulgaris. Gas chromatography-mass spectrum analysis analysis of Thymus vulgaris revealed the existence of the Erythritol, γ-Terpinene, Octan-2-one, 3, 6-dimethyl-, DL-Leucine, N-glycyl-, Trans-2-Caren-4-ol, Dithiocarbamate, S-methyl-, N-(2-methyl-3-oxobutyl)-, 5-Caranol, trans, trans-(+)-, Phenol, 2-methyl-5-(1-methylethyl)-, Phenol, 2-methyl-5-(1-methylethyl)-, acetate, (5β)Pregnane3, 20β-diol, 14α, 18α-[4-methyl-3-oxo(1-oxa-4-, β-Bisabolene, 1-Chloro-1-n-decyloxy-1-silacyclopentane, 7-epi-trans-sesquisabinene hydrate, (−)-Spathulenol, Caryophyllene oxide, 1-Heptatriacotanol, Ppropiolic acid, 3-(1-hydroxy-2-isopropyl-5-methylcyclohexyl)-, Naphthalene, 1, 2, 3, 4, 4a, 5, 6, 7-octahydro-4a-methyl-, Paromomycin, Gibberellic acid, Phytol, 2, 7-Diphenyl-1, 6-dioxopyridazino[4, 5: 2’, 3’]pyrrolo[4’, 5’-d] pyrida, Androst-4, 6-dien-3, 11, 17-trione, 9-mercapto-, Ricinoleic acid, 4a, 10a-Methanophenenthren-9β-ol, 11-syn-bromo-1, 2, 3, 4, 4a and (+)-γ-Tocopherol, O-methyl. Top Keywords Thymus vulgaris, Anti-diabetic, α-amylase, GC-MS, α-glucosidase, Secondary metabolites. Top |