Protective effects of braintox (Aroma oil mixture) on memory impairment and arc and fos expression in amyloid Beta-Mice Model Leem Kanghyun* College of Korean Medicine, Semyung University, Jecheon, 27136, Republic of Korea *Corresponding Author: Kanghyun Leem Professor, College of Korean Medicine, Semyung University, Republic of Korea Email: lkh@semyung.ac.kr
Online published on 16 October, 2018. Abstract Objectives The target of this paper is to confirm the protective effect of the aroma oil-mixture (Braintox) on cognitive dysfunction in the traditional knowledge of Oriental medicine.. Method The amyloid β (Aβ) toxicity is known to induce the neuronal dysfunction and death. The Aβ intracerebroventricular injection animal model was used to make a cognitive dysfunction. The step through latency was measured via passive avoidance behavioral test. The Arc and Fos gene expression was calculated via real-time qPCR. The acetylcholinesterase inhibitory effect of Braintox was measured.. Findings The Aβ intracerebroventricular injection made a cognitive dysfunction and significantly reduced the time of step through latency (time) compared to sham group. Braintox treatment significantly increased the latency time compared to Aβ-injected mice. Braintox treatment significantly increased Arc and Fos gene expressions in hippocampi. The reduction of acetylcholinesterase activity could be effective to help the cholinergic neurons. Accordingly, the antiacetylcholinesterase assay was performed and the Braintox significantly reduced the acetylcholinesterase activity.. Improvements The present results proposed that Braintox could have beneficial effects on the cognitive dysfunction. Its effects might be explained by the mechanisms including Arc, Fos and antiacetylcholinesterase activity. Top Keywords Alzheimer's disease, Amyloid beta, Braintox, Dementia, Learning and memory. Top |