Clinical studies on the dental caries prevention effects of the ability of Weissella cibaria CMU to adhere to the oral cavity Park Hae-Ryoung1,*, Kim Hye-Jeong2, Kang Mi-Sun3 1Assistant Professor, Division of Liberal Arts and Teacher Training, Kwangju Women's University, South Korea 2Researcher, Department of Pediatric Dentistry, School of Dentistry, Chonnam National University, South Korea 3Head, Department of Research Institute, Oradentics Inc., South Korea *Corresponding Author: Hae-Ryoung Park Assistant Professor, Division of Liberal Arts and Teacher Training, Kwangju Women's University, South Korea E-mail: hrpark@kwu.ac.kr
Online published on 16 October, 2018. Abstract The purpose of this study is to investigate the effects of diet containing Weissella cibaria CMU (oraCMU) on Streptococcus mutans causing dental caries. The m-PHP index was Modified Patient Hygiene Performance Index. As a measuring method, Modified patient hygiene performance index was measured to quantify oral hygiene condition among 2 subject groups consisting of W. cibaria CMU (oraCMU), a commercial product containing W. cibaria CMU and the same commercial product but removing W. cibaria CMU as a placebo control. Each group had 25 subjects. Unstimulated saliva were collected and analyzed by real-time polymerase chain reaction (RT-PCR). The measurement of Modified patient hygiene performance index was performed every 2 weeks starting from the first day of the experiment. And to know the effects of post-diet, its measurements were continued every 2 weeks for 4 weeks of a post-diet period. Data were analyzed by One-way ANOVA was carried out for the significance test between groups. Scheff's post hoc was used to determine variance homogeneity differences between the mean values (p < 0.05). Modified-patient hygiene performance (m-PHP) index were significantly reduced in the Weissella cibaria CMU (oraCMU) compared to the removing W. cibaria CMU (oraCMU) as a placebo control, with significant differences between groups (p＜0.05). Our survey confirms that there are many individual subjects who believed that oral health could be maintained with diet of W. cibaria CMU (oraCMU). Specifically, there was a statistical significance difference between Weissella cibaria CMU (oraCMU) and placebo control groups during both of the 2-weeks and 4-weeks (p＜0.05). Streptococcus mutans levels significantly decreased in the W. cibaria CMU (oraCMU) compared to the removing W. cibaria CMU as a placebo control during the intervention. The oral colonization of W. cibaria CMU (oraCMU) was also observed. These results suggest that W. cibaria CMU (oraCMU) were serves as oral probiotics, to prevent dental caries and good oral health. Our results represented that Weissella cibaria CMU (oraCMU) diet were significantly suppression of biofilm formation on the surface enamel in terms of oral hygiene. This study would be used as basic data for future research in the development of oral hygiene products. Top Keywords Biofilm, Dental caries, modified patient hygiene performance (m-PHP) index, Streptococcus mutans, Weissella cibaria CMU (oraCMU). Top |