Extract of elicited kaffir lime (Citrus hystrix DC.) cells suspension by Saccharomyces cereviseae H. and Its citotoxicity against T47D cells Priyanto Dwi1, Tunjung Woro Anindito Sri2, Indriyanto Ari2 1Health Research and Development Center, Banjarnegara, Central Java, Indonesia 2Faculty of Biology, Gadjah Mada University, Yogyakarta, Indonesia Online published on 5 November, 2018. Abstract The previous study indicates that kaffir lime leaves extract potentially used as a cancer drug. Bioactive materials in the extracts have a cytotoxicity effects on cervical cancer cells, neuroblastoma and breast cancer, and increase the percentage of cells apoptosis. Other previous research also succeeded in developing callus from explants of kaffir lime seeds. The usage of plant biotechnology with kaffir lime cells has been able to produce various bioactive compounds that are similar to their original conditions. Objective of this study is to determine cytotoxic activity of elicited Citrus hystrix DC. cell suspension using Saccharomyces cereviseae H. The 16 days-old suspension culture reached the stationary phase was treated by the elicitor at concentrations of 1 and 10 mg/ml then harvested on the 2nd and 4th days after elicitation. Ethyl acetate extract of elicited kaffir lime cells was obtained by macerations methods. Identification of bioactive compounds in the extract was performed by GC-MS while anticancer potency was examined through MTT method on T47D cells. The study shows that extract of kaffir lime cell suspension elicited by 10 mg/ml S. cereviseae H. and harvested on day 4 after inoculation was the most toxic to T47D cells (IC50 value was 59,82 μg/ml). All treatment succesfully stimulate the culture to synthesize potentially anticancer secondary metabolites: germacrene A, alpha-pinene, beta-selinene, alpha-selinene, beta-ocimene, benzene, cadinene and globulol. Therefore, we assumed that the last three compound increased cytotoxic effect significantly. Top Keywords Citrus hystrix DC, Saccharomyces cereviseae H, elicitation, cytotoxicity. Top |