Development and Validation of Lercanidipine Hydrochloride and Atenolol by Using RP-HPLC and UV Spectroscopy Bansode Priyadarshani S.1, Ranawat Mahendra Singh2, Kamble Ravindra2, Chauhan Chetan Singh2 1Faculty of Pharmacy, Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, India. E-Mail: priya.s.bansode@gmail.com 2Bhupal Nobles’ College of Pharmacy, Udaipur, Rajasthan, India Online published on 21 March, 2015. Abstract Lercanidipine hydrochloride (LCD), a dihydropyridine calcium channel blocker is used in the treatment of hypertension. Atenolol (ATN), a cardio selective β-blocker is used as antihypertensive, antianginal and antiarrhythmic. The proposed method was development and validation of UV-Spectrophotometric and RP-HPLC for determination in bulk and tablet dosage form. In UV Spectroscopic method, LCD showed λmax at 237 nm in 50% v/v acetonitrile. The RP-HPLC analysis was performed by using a mobile phase of methanol: water (95:5 v/v), at a flow rate of 1.0 ml/min. By comparing validation results of both the methods, UV-spectrophotometric method is found to be simple, economical and rapid and RP-HPLC method were found to more precise, accurate, rugged and robust. Top Keywords Atenolol, Lercanidipine hydrochloride, UV-spectrophotometr, RP-HPLC. Top |