Efficacy of a novel β-mannanase on intestinal digesta viscosity in broiler chickens fed diets with high levels of guar meal (Cyamopsis tetragonoloba) Fickler A.1, Kore K.1,*, Matthews A.2, Moore D.2, Mandal A.B.3 1BASF SE, Carl-Bosch-Strasse 38, 67056Ludwigshafen, Germany 2Colorado Quality Research, INC., 400 East County Road 72, Wellington, Colorado, 80549 3ICAR-Central Avian Research Institute, Izatnagar243 122 (UP), India *Corresponding author Email: kumar.kore@basf.com
Online Published on 15 January, 2024. Abstract An experiment was conducted to evaluate the efficacy of novel endo-1,4-ß-mannanase in male Cobb 500 broiler chicken fed with a high level (7.5%) of guar meal as a replacement of soybean meal. Total of 1,050 broiler chicks (day old) were divided into 5 groups with 21 birds x 10 replicates in each treatment group. Two basal diets were formulated: positive control (PC/T1) containing maize - soybean meal (SBM) based standard broiler diet and negative control (NC/T2) containing maize - SBM based iso-caloric and iso-nitrogenous broiler diet with inclusion of 7.5% guar meal replacing SBM. Treatments T3, T4, and T5 diets were based on NC/T2 diet supplemented with mannanase at three different doses 400, 800 and 1200 TMU/kg, respectively. Each diet was prepared for starting (0-14d), growing (14-28d) and finishing (28-42d) growth phases of broiler chicken. Ileal digesta samples were collected from two birds from each replicate (20 birds per treatment) on 28d and 42d of age of birds. The inclusion of guar meal had a significant negative effect (P<0.0001) on feed intake, feed conversion ratio, as well as body weight gain in broilers who received the diets containing guar meal compared to the positive control group. However, supplementation of mannanase significantly improved performance (P<0.0001) compared to broilers fed with the negative control diet. In addition, broilers received the negative control diet (T2) showed significantly (P<0.0001) higher digesta viscosity on both sampling days (day 28 day 42) compared to the positive control (T1) without guar meal and the mannanase treatments (T3, T4, T5). Thus, mannanase, independent of the applied dosage, was able to reduce viscosity of digesta in birds fed guar meal containing corn-soybean meal-based broiler diets and the results were comparable with positive control group fed without guar meal. In conclusion, mannanase (Natupulse® TS) proved effective in reducing digesta viscosity and thus, is able to reduce the negative effects of dietary guar meal on broiler growth performance. Top Keywords β—mannanase, Chicken, Guar meal, Soybean meal. Top |