Comparative study of molecular variations in Mycoplasma synoviae field isolates from common respiratory infections of poultry in Andhra Pradesh Thopireddy Nagendra Reddy*, Bollini Sreedevi, Nagaram Vinod Kumar Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati–517502 *Corresponding author Email: tnreddy32@gmail.com
Online Published on 15 January, 2024. Abstract Samples collected from 19 Mycoplasma suspected poultry farms in A.P. including 228 pooled cloacal, nasal and tracheal swabs from the ailing birds and 152 tracheal tissues, 152 lung tissues and 76 pooled oviduct samples from the dead birds. Molecular detection was done by targeting vlhA gene in PCR. The homology comparison between MS field strains (TH11, TH13, MSTH17 and MSTH18), vaccine strain (MS vac, (MS-H strain)) and other gene bank sequences revealed 99.81% to 100% homology between field, vaccine and published sequences. In MS clinical isolates were showed variations at 24th to 338th nucleotide positions and unique mutations observed at C70T, T93C, T99G, C101A, T132C, G138A, C181T, T199G, G203A, A263C, C266A, A292G and A117G leads to amino acids sequence variations at 9th to 112th positions in field vlhA genes. The phylogenetic tree revealed that MS vaccine isolate was closely related to T2/3XvlhA (AY907704) Australian strain, the field TH13 and the field MSTH17 was closely related and all the field strains TH11, TH13 and MSTH17 were evolved from MSTH18 field strain and also the GenBank isolates also evolved from MSTH18. Top Keywords MS, VlhA gene, PCR, Sequencing, Phylogenetic analysis. Top |