Expression of toll-like receptors in caseous lymphadenitis infected sheep Kaur Navneet1, Meena A.S.2, Kumar Rajiv2,*, Khare Neeraj1, Sharma R.C.2 1National Institute of Medical Sciences University, Jaipur-303 121, Rajasthan 2ICAR-Central Sheep and Wool Research Institute, Avikanagar-304 501, Rajasthan *E-mail: rajivbiotech028@gmail.com
Online Published on 12 February, 2024. Abstract Caseous lymphadenitis (CLA) is a chronic disease of sheep and goats, characterized by abscess formation in lymph nodes, mainly the parotid and sub-mandibular. This disease is caused by Gram-positive bacteria Corynebacterium pseudotuberculosis. Toll-like receptors (TLRs) are a type of pattern recognition receptors which primarily recognize pathogen associated molecular patterns (PAMPs) and induce innate immune system. The expression patterns of TLRs in CLA infected sheep have been investigated. RNA was extracted from blood samples of CLA infected and healthy sheep. Complementary deoxyribonucleic acid (cDNA) copies were synthesized and real-time polymerase chain reaction (PCR) was conducted. TLR-4 and TLR-5 genes were significantly (P<0.05) up-regulated and TLR-1 and TLR-10 genes were significantly (P<0.05) down-regulated in CLA infected as compared to healthy sheep. The study suggested that an immune-mediated pathway seems to play role in CLA infection. Top Keywords Caseous lymphadenitis, Corynebacterium pseudotuberculosis, Real-time polymerase chain reaction, Toll- like receptors. Top |