Bio-Pesticidal efficacy of purified fractions of Solanum nigrum (L) whole plant extracts on insect Tribolium castaneum (herbst.)-A red flour beetle Namdeo Ritu1, Chauhanb Rajendra2, Gound M.L.1, Shende Kishor3, Soni Kapil K.1,* 1Department of Biosciences, Barkatullah University, Bhopal, Madhya Pradesh, India 2Department of Zoology, Govt. Motilal Vigyan Mahavidyalaya, Bhopal, M.P, India 3Department of Biotechnology and Bioinformatics Centre (Sub-DIC), Barkatullah University, Bhopal, Madhya Pradesh, India *Corresponding author
Online published on 24 May, 2018. Abstract Since many centuries peoples are struggling to protect stored grains which are fed by insects. Synthetic insecticide has led many problems such as environmental disturbances, pest resurgence, pest resistance to pesticides and lethal effects on non-target organisms including man. Therefore, natural products isolated from plants have become as potential sources of new pesticides. In the present study, the insect Tribolium castaneum (Herbst) is a damaging pest of wheat grains and its flour. Looking to the economic importance of host plant Triticum aestivum as edible crop that is being fed by the pest Tribolium castaneum, it was thought important to develop bio-pesticides of plant origin. Plant Solanum nigrum (L.) after identification and authentication from RHMD, New Delhi, collected from the Arera colony area of the Bhopal and shade dried and pulverized to get powder which was extracted in different solvents through Soxhletion and percentage yield was noted down 1.67% in benzene. Obtained extract were fractionated by TLC and RF value were measured viz. 0.12, 0.40, 0.48, 0.89 in Petroleum ether: Toluene: Ethyl acetate (50: 30: 20) and Rf value 0.59, 0.68, 0.78, 0.90 were measured in Chloroform and methanol (50: 50) and column chromatography was also sought to get purified fraction which was applied on adult insects Tribolium castaneum L. Results showed that purified fractions of Solanum nigrum benzene extract at 100% concentration was reported with 86.67±15.28% mortality that is compared with standard drug Parad Tikdi which showed 6.67±11.5% mortality and LC50 value was reported 51.50% and upper and lower values at 95% confidence interval are 62.44% and 40.82%, respectively Top Keywords Bio-pesticides, Bio-insecticides, Phyto-chemicals, Mortality, Solanum nigrum, Tribolium castaneum. Top |