Utility of line probe assay for identification of Mdr-Tb and ntm in smear positive sputum samples from a tertiary care hospital of Western U.P. India Nudrat Sana1,*, Farooq Umar2, Mohan Shivendra1 1Department of Microbiology, Teerthankar Mahaveer Medical College, Moradabad, Uttar Pradesh, India 2Department of Microbiology, Teerthankar Mahaveer Medical College, Moradabad, Uttar Pradesh, India *Corresponding author
Online published on 24 May, 2018. Abstract Detection of the MDR & XDR status and NTM identification from smear positive sputum samples by Line Probe Assay Study was performed on smear positive sputum samples collected from DOTS centre at TMMC & RC, Moradabad during period of July 2015 to March 2017. Samples were decontaminated by Pettrof method and then DNA was extracted by using heating method, and then were subjected to PCR using Applied Biosystem Veriti 96 well Thermal Cycler and Line Probe Assay by using Geno Type MTBDR plus on Twincubater of HAIN life sciences. Total 200 smear positive sputum samples were processed by line probe assay, in which 16 were MDR, 2 were XDR and 10 were detected as NTM. In this study, we concluded that, on routine basis we should use line probe assay for drug sensitivity of M.tuberculosis cases, so that appropriate treatment can be given on time to the patients. Top Keywords MDR-TB, XDR-TB, NTM, PCR, LPA. Top |