Purification and characterization of catechol 1, 2-dioxygenase from Pseudomonas putida Strain PS-I Singh Ashok Kumar1, Singh Alok Kumar2,* 1Department of Boatny, S. R. T. C. P.G. College, Utraula, Balrampur, Uttar Pradesh, India 2Department of Botany, Harish Chandra PG College, Varanasi, Uttar Pradesh, India *Corresponding author
Online published on 24 May, 2018. Abstract Pseudomonas putida Strain PS-I utilized 4-chlorobenzoic acid as a sole source of carbon and energy, degrading it through the ortho ring cleavage pathway. They synthesized the enzyme catechol 1, 2-dioxygenase which is unique in its properties to transform chlorocatechol of 4-chlorobenzoic acid in to chloro cis-cis muconic acid, while catechol 1, 2-dioxygenase is only efficient degrader of catechol in to cis-cis muconic acid. The enzyme was purified to homogeneity by ion exchange and gel filtration chromatography from culture supernatant. The purified native had an estimated molecular mass 55 kDa and it is composed of two sub units each of 25 kDa determined by SDS-PAGE. Top Keywords 4-chlorobenzoic Acid, Catechol 1, 2-dioxygenase, Pseudomonas putida Strain PS-I. Top |