Indian Journal of Scientific Research
Year : 2019, Volume : 9, Issue : 2
First page : ( 61) Last page : ( 62)
Print ISSN : 0976-2876. Online ISSN : 2250-0138.
Article DOI : 10.32606/IJSR.V9.I2.00010

Detection of anthocyanins of flower extracts of desmodium gangeticum (L) DC

Prabhat V.K., Kumar Nandjee

Department of Botany, Magadh University, Bodhgaya, Bihar, India

Online published on 6 September, 2019.

Abstract

The present paper deals with the detection of anthocyanins of flower extract of Desmodium gangeticum (L) DC. Anthocyanins are glycosidic pigments usually found as essential constituents of flower petals or coloured leaves. They are antioxidant, antiviral and antimutagenicity and play important role in biological system. 2.00 g of flower petals of Desmodium gangeticum (L) DC were dissolved in 2M Hcl in a test tube and heated 100°C for 5 mts. The solution wascooledand washed with ethyl acetate to remove the flavonoids followed by filtration. The filtrate was further heated with amyl alcohol at 80°C for 5 mts followed by filtration. The filtrate contained anthocyanin so the filtrate was spotted on Whatman's filter no 1 and was placed in the chromatographic jar for separation of anthocyanins by using the solvents:

1. Forestall conc-HCl. CH₃COOH.H₂O-3: 6: 1

2. Formic acid conc-Hcl. HCOOH.H₂O-2: 5: 3

The coloured spots appeared on the chromatogram indicated anthocyanin. From the colour of the individual spot, anthocyanins were differentiated and detected. The Rf value of the marked spots was noted and different anthocyanins were confirmed by comparing the respective Rf value. The results confirmed three types of anthocyanin namely cynidin, peonidin and petunidin.

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Keywords

Desmodium gangeticum (L) DC, Forestall Conc. and Formic Acid Conc.

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