Pluronic F-127-based Shorea robusta resin powder hydrogel revamped the anti-oxidant status and countered sustained inflammation to facilitate healing of diabetic wounds Patel Madhuribahen Ratishkumar1, Saddam Abdul1, Sharma Anshuk2, Singh Wangkheirakpam Ramdas1, Golaviya Akash3, Kumawat Sanjay1, Singh Thakur Uttam4, Kumar Dinesh5,† 1PhD scholar, Division of Pharmacology and Toxicology, ICAR-Indian Veterinary Research Institute, Izatnagar- 243 122 (Uttar Pradesh) 2Scientist, Division of Pharmacology and Toxicology, ICAR-Indian Veterinary Research Institute, Izatnagar- 243 122 (Uttar Pradesh) 4Senior Scientist, Division of Pharmacology and Toxicology, ICAR-Indian Veterinary Research Institute, Izatnagar- 243 122 (Uttar Pradesh) 5Principal Scientist, Division of Pharmacology and Toxicology, ICAR-Indian Veterinary Research Institute, Izatnagar- 243 122 (Uttar Pradesh) 3PhD Scholar, Department of Microbiology, Kamdhenu University, Anand. †Corresponding author; E-mail: dineshks17@gmail.com
Online Published on 20 May, 2023. Abstract Adverse outcome related to wound healing in diabetic animals has been widely documented and chronic wounds create a serious financial burden on the pet owners. Hence, there is utmost need of a safe, cost-effective, and pragmatic wound healing modality. Considering the beneficial effects of Pluronic F-127 (PF-127) and Shorea robusta resin extracts in wound healing, the healing efficacy of hydrogels based on easily available S. robusta resin powder (SRRP) and PF-127 in excisional wound model in diabetic rats was evaluated. Wounds were created in 24 rats and they were divided randomly into 4 groups. Group I (20% w/v PF-127 gel) served as vehicle control while groups II, III, and IV topically received 1%, 1.5%, and 2% SRRP hydrogels, respectively, b.i.d. for 19 days. 2% SRRP hydrogel markedly improved wound closure with simultaneous reduction in pain perception, as compared to vehicle control. It also countered inflammation as evidenced by a marked fall in tumour necrosis factor-α (TNF-α) levels with a sharp, significant rise in interleukin-10 (IL-10) and hypoxia inducible factor-1α (HIF-1α) levels. Further, a significant improvement was observed in the levels of hydroxyproline and glucosamine along with the activities of enzymatic and nonenzymatic antioxidants. 2% SRRP hydrogel appeared to have good potential in healing diabetic wounds owing to its analgesic, anti-oxidant, and anti-inflammatory properties. Top Keywords Antioxidants, Diabetes, Inflammation, Pluronic F-127-based hydrogel, Shorea robusta resin, Wound healing. Top |