In-silico Designing and Testing of Multiprotein Vaccine Construct Against Cow Milk Allergy Sukhija Nidhi1, Kanaka K.K.1,*, Jaglan Komal2, Raj Chethan3, Sukhija Sona4, Vidhyasagar5 1ICAR-National Dairy Research Institute, Karnal, Haryana, India 2Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India 3ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., India 4Government Medical College, Haldwani, Uttarakhand, India 5Veterinary College, Bidar, KVAFSU, Karnataka, India *Corresponding author: KK Kanaka; E-mail: kkokay07@gmail.com
Online Published on 22 December, 2023. Abstract Cow’s milk allergy is a common diagnosis in infants and children, and it manifests as a hypersensitivity reaction to the allergenic proteins in cow milk. Cow milk contains about 20 distinct protein components. The primary allergens are casein protein (alpha-s1-, alpha-s2-, beta-, and kappa-casein) and whey protein (alpha-lactalbumin and beta-lactoglobulin). There are several allergy treatments available, but they are just temporary. Developing a vaccine against milk allergy may appear utopian. Epitopes were found and exploited to build a multiprotein vaccine in this immunoinformatics study. Immunoinformatics techniques were used to predict T- and B-cell epitopes. Adjuvants were used to boost the vaccine’s antigenicity. The created vaccine design was shown to be soluble, antigenic, non-allergenic, and non-toxic. Highlights Cow milk contains around 20 proteins which may cause allergy. Immunoinformatics approach is used to design vaccine. Allergens are identified and vaccine is constructed and tested in-silico.
Top Keywords Allergy, Epitopes, Immunoinformatics, Milk proteins, Vaccine. Top |